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Briefly, brains were dissected into 2.5% paraformaldehyde, 1% glutaraldehyde, 1% sodium metabisulfite in 0.1 M cacodylate buffer, pH 7.2, microwaved with a Pella research-grade oven (# 3450, with variable power controller #3430 and cold spot) with chilling plate at 18°C and power setting #2 for 2 min on/2 min off/2 min on/2 min off, and then fixed overnight on a shaker at 4°C.
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Spoon fish neatly onto chilled plates; garnish with cilantro.
Glass may be poured in graphite or steel molds or, alternatively, rolled (using a metal roller) into thin flakes while being poured onto a steel or aluminum chill plate.
However, when the melt undercooled below Tp was dropped onto the copper chill plate with high thermal conductivity, YIG solidified around the chill plate as a primary phase.
On the other hand, for the undercooled melt dropped onto the quartz glass chill plate with low thermal conductivity, YIP primarily solidified.
These results suggest that the high cooling rate achieved by the copper chill plate prevented the nucleation of YIP and led to the direct growth of YIG, which is explained by the phase selection based on the competitive nucleation rate.
To compensate for a raw dish's lack of aroma -- "one of the things that's so enjoyable about eating," Mr. Trotter said -- he serves dishes at room temperature (never chilled) on plates that have been warmed in the oven, so that the flavor "blooms a little".
Traditionally you would make these simply by making the frittatas, leaving them a little runny on the top and stacking them, then finishing the stack in the oven or just weighting it with a plate and chilling.
Inoculum was obtained by adding 10 ml ddH2O to each plate and chilling for 2.5 3 hr at 4°.
Signal transduction was stopped by chilling the cell culture plates on ice and adding ice-cold PBS.
The first 15 were devoted to the electromagnetic assault on my axilla, then a hydro-ceramic "cooling plate" chilled my skin for the next 25.
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