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Cells were observed and captured by a Zeiss Axioplan microscope equipped with a chilled video charge-coupled device camera (C4742-95; Hamamatsu Photonics, Bridgewater, NJ).
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Parasites were stained with YOYO-1 fluorescent nucleic acid staining reagent (Molecular Probes, Inc .. Bright field and fluorescent images were collected with a chilled CCD video camera (Model C5985, Hamamatsu Photonic Systems, Bridgewater, NJ).
When another friend casually plopped their quiet newborn onto a couch cushion and then sat down at their computer sipping tea watching YouTube videos while their baby "chilled," my head fell into my hands in disbelief.
The setup at Pikes – the spot where Wham! filmed their video for Club Tropicana – is extremely chilled by day, but in the evening 1,000-plus 1,000-plus descend on it for some club action, including a 2am set by Elba.
The spot also features local art and video projections, a 2,000-bottle 2,000-bottleand a kitchen, ideal for late-night snacking, that serves dishes like chilled smoked mussels.
Chilled perfection!
Chilled squid salad.
Chilled Imperia vodka!
A chilled lager?
Chilled seafood assortments, $24 to $88.
"Chilled to the Zuckung".
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