Exact(2)
The bands were transferred overnight from gels to PVDF membranes using chilled transfer buffer at 4°C.
Once chilled, transfer to a suitable storage container, and keep refrigerated.
Similar(58)
9 Glass field vials and zip lock bags were chilled and ticks transferred to labeled cryotubes.
Give the lightly jellied juice a brief stir to break it up, and transfer into chilled bowls.
Wearing purple latex gloves, the science party grips buckets of chilled water, ready to transfer the cargo to the walk-in refrigerator, where the microbes, worms, snails and other creatures of the deep will be much more comfortable.
Furthermore, fruit decay decreased in tomato treated with cinnamon or eucalyptus oil (0.05 0.5 mL/L) vapours and transfer to chilled air [ 17].
Recent studies investigated various methods for inducing mild hypothermia in awake volunteers or awake patients, such as infusion of cold saline [ 21, 22], surface cooling with circulating chilled water through energy transfer pads placed on the patients' skin [ 14, 23, 24], or endovascular cooling catheters [ 4, 15, 25- 28].
The sensory organs were then transferred to chilled Medium-199 and the sensory epithelia (consisting of sheets of hair cells and supporting cells) were separated from the associated stromal tissue using a 27-gauge needle.
Part of the brain including the amygdala was rapidly removed and transferred into chilled oxygenated saline of the following composition (mM): KCl, 2.4; MgSO4, 10; CaCl2, 0.5; piperazine-N,Ń'-bis ethanesulphonic acid) (piperazine-N,Ń'-bis ethanesulphonic95 (piperazine-N,Ń'-bis ethanesulphonic
To generate protein extracts for immunoprecipitation, 100 200 adult flies were frozen in liquid nitrogen, ground to a fine powder using a chilled mortar and pestle, transferred to a Dounce homogenizer and homogenized in 1.5 ml of lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, 10% glycerol, 10 mM NaF, 1 mM Na3VO4, 100 µg/ml PMSF, Sigma protease inhibitor cocktail (Sigma #P8340)).
The effluent was collected into sterile syringes and transferred into chilled centrifuge tubes with FCS.
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