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Brains were removed and chilled rapidly on ice.
The mouse brains were removed and chilled rapidly on ice.
Samples were harvested periodically for transcriptome and proteome analysis and chilled rapidly in dry ice/ethanol bath before centrifugation.
Harvested cells (108 at each time point) were mixed immediately with 10% buffer-saturated phenol in ethanol (1/10 volume) and chilled rapidly to 4°C [21].
Four microgram total RNA from each sample was denatured at 70°C for 5 min and chilled rapidly on ice.
After stuffing and linking, fresh sausages should be dried at room temperature for a short time and chilled rapidly.
Similar(54)
Once obtained, the sample was chilled and rapidly transported to the laboratory.
In deep M. semimembranosus (SM) a temperature of 10 °C was achieved within 11.5 h in rapidly chilled carcasses and within 14 h in slowly chilled carcasses.
A temperature of 10 °C was achieved in the centre of M. longissimus dorsi (LD) within 3.5 h in rapidly chilled carcasses and within 8 h in slowly chilled carcasses.
CSF was collected into chilled, polypropylene tubes, rapidly frozen, and maintained at –70 °C until analyzed.
An exponentially growing culture (1 litre, O.D.540 nm 0.3 O.U). was rapidly chilled and collected by centrifugation as described above.
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