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After intraocular injection, larvae were retrieved by placing the dish on a chilled ice pad to melt the CyGEL and immediately transferred to Embryo Medium at RT for recovery.
The isolated EVs were incubated at 99°C for 20 min and then treated with chilled ice for 20 min to denaturalize proteins in EVs.
All tubes were immediately stored in a chilled ice box and transferred on the same day to the laboratory at BPKIHS, where 180 μL of each sample was transferred to a tube containing 180 μL of AS1 buffer (catalog no. 1006243; QIAGEN, Venlo, the Netherlands), mixed well, and stored at room temperature.
Pour in the chilled ice cream mix.
A chilled ice or granita as an intermezzo will prepare your guests' palates for the next course.
Pour the chilled ice cream base through a fine mesh strainer.
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The spiky-looking "leafy plant" cupcake requires chilled icing and many short squeezes of the pastry bag.
Is it all lifeless, deep-chilled ice, or is some of it liquid and thus capable of sustaining life?
Cells from each individual well were collected in a pre-chilled ice-water cytometric tube, washed and processed essentially as detailed previously [40].
The striatum, and SN were dissected out on a chilled glass dish on ice.
Strain into a chilled wine glass over ice, top with prosecco, and garnish.
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