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I do not follow the aforementioned "chill protocol".
For those residents of Vancouver who follow the "chill" protocol, it's not just about being a free spirit who walks around downtown without shoes.
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Cancer mastectomies may not be optimal for whole-breast studies because of the necessity for immediate histological examination to determine treatment, but the Egan protocol [ 13] (chilling and sectioning a mastectomy breast prior to fixation) might allow thick-section study of even cancer-containing breasts without compromising their diagnostic evaluation.
However, we will administer prophylactic Abx, as per protocol, and will ask any subject with fever or chills after the procedure to seek medical attention immediately.
For chromosome preparations, the cells were harvested following conventional cytogenetic protocol of hypotonic treatment (75 mM KCl) and freshly prepared chilled 3 1 acetone:methanol fixation.
Effect of methanol at molecular level during chilling was reported here first time which could add new parameter in selection of cryoprotectant while designing cryopreservation protocol.
Chill, chill.
Just chill.
Major chill.
Chill out.
Pretty chill.
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