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The resin with ABI ligand was then used to separate IgY from immunized chicken serum.
Dexamethasone (Dex, 1 μM) stimulated differentiation in 2.5 or 10% chicken serum (P<0.05).
Insulin, Dex and 2.5% chicken serum stimulated enzymatic differentiation to the extent of 10% chicken serum, but heparin (10 U/ml) addition, in combination with insulin and Dex was necessary to stimulate lipid filling of adipocytes.
Insulin (100 nM) stimulated expression of citrate lyase and sn-glycerol-3-phosphate dehydrogenase relative to lactate dehydrogenase in the presence of 2.5% chicken serum (P<0.05), but not with 10% chicken serum (P>0.05).
The reaction of IgG-Blue-SiNPs with S. pullorum was inhibited by 1 100 dilution of positive chicken serum.
The sensitivity of the Western blot assay was not sufficient to permit the direct detection of leptin in chicken serum or plasma.
The cultural medium (2 l) for LSCC-RP9 cells included 11.9 g McCoy's 5A medium modified (Sigma Co., USA), 13.8 g Leibowitz L-15 medium (Sigma Co., USA), 20 % chicken serum, 10%% fetal bovine serum, and 5%% tryptose phosphate broth.
Fetal bovine serum (FBS) and chicken serum were from Hyclone.
It has been shown previously that cells without clathrin die under low chicken serum supplementation [17].
Wettey et al. showed that under low chicken serum conditions DKO-S cells die without clathrin.
In 100% chicken serum, no differences were observed between plasmid-containing and plasmid-lacking strains.
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