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Burns, A. J. & Delalande, J. M. Neural crest cell origin for intrinsic ganglia of the developing chicken lung.
BACKGROUND: New branches within the embryonic chicken lung form via apical constriction, in which epithelial cells in the primary bronchus become trapezoidal in shape.
Viral analysis showed high viral titres in chicken lung, caecal tonsil, liver and heart tissue.
After 10 serial chicken lung passages of the QA23 virus, we prepared a stock of the virus by inoculating the 10th passaged chicken lung homogenate into embryonated chicken eggs; the virus was designated QA23CkA10 (Table 2).
The chicken-adapted (QA23CkA10) virus was isolated from the 10th chicken lung passage of the QA23 virus.
During infection with H5N1, chicken lung tissue showed iNOS staining in the respiratory epithelium and the surrounding sub mucosa.
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In addition, all six H9N2 isolates were able to replicate more efficiently in mouse lungs than in chicken lungs (Table 2).
The experimental trials in chickens showed that the six H9N2 isolates tested herein were able to replicate in chicken lungs, but did not produce observable clinical disease or death over a 21-day observation period.
In the libraries of chicken lungs, 98,849 and 179,549 reads were obtained from infected and non-infected lungs, respectively.
A total of 278,398 and 340,726 filtered high quality reads were obtained from chicken lungs and tracheae, respectively (Table 1).
Of the 474 distinct Gallus gallus (gga) miRNA entries in miRBase [ 19- 22], 377 miRNAs were identified in chicken lungs and 149 miRNAs in chicken tracheae.
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