Chicken full-length cDNA sequences were downloaded from the UMIST www site (Sept 2004).
The average number of reads per chromosome is consistent with the chicken chromosome lengths, with the exception of the Z chromosome, where more reads are observed than expected.
A local version of RepeatMasker [ 20] that contained either (i) a specific library of known chicken full-length OR nucleotide sequences [ 13] or (ii) a (non-OR) G-protein coupled receptor (GPCR) sequence library consisting of 327 chicken sequences [ 14] was used to distinguish OR genes from non-OR GPCRs.
Each cDNA sequence, depending on the source of the probe design, was downloaded from ENSEMBL or the NCBI, and, as most of the cDNA sources were Expressed Sequence Tags, full length chicken mRNAs were sought by BLAST searching each of them against the Refseq chicken nucleotide database.
The full length chicken mRNA sequences ranked good and reasonable were then used in a RBH analysis.
The feature-length Chicken Run was an extended spoof on prisoner-of-war pictures, most especially The Great Escape, set on a poultry farm.
He won Oscars for best animated short three years running with Creature Comforts, The Wrong Trousers and A Close Shave, before going on to co-direct with Aardman co-founder Peter Lord the feature-length Chicken Run in 2000.
Previously, we developed pcDNA-CCOL2A1, a novel therapeutic DNA vaccine, which encodes the full-length chicken type II collagen sequence, and demonstrated that the efficacy of this vaccine for treating rheumatoid arthritis was comparable to that of the current "gold standard" treatment, methotrexate.
The full-length chicken α-subunit cDNA was kindly provided by Dr. Patricia A. Johnson (Cornell University, Ithaca, New York, USA) and subcloned into the first multiple cloning site of the inhibin A bicistronic plasmid replacing the human α-subunit cDNA.
MAGp72_pXM plasmid containing full-length rat L-MAG was a kind gift from Rainer Hillenbrand, Basel, Novartis Institutes for BioMedical Research [28], MOG_pCMV containing full-length human MOG alpha variant was provided by Markus Reindl, Innsbruck Medical University and N-CAM_pBK-CMV containing full-length chicken N-CAM180 was given to us from Jozsef Kiss, Geneva, University Medical Center.
