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Preparation of chicken core histones and nucleosomes was performed as described previously [ 63].
Reactions were performed using 1 μl concentrated enzyme, 5 μg purified chicken core histones, 0.1 μl C-acetyl-coenzyme A (AcCoA) (50 mCi/mmol) in 15 μl 1 × buffer (50 mM sodium phosphate pH 7.4, 15 mM 2-mercaptoethanol, 10% glycerol) for 15 min at 37°C.
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Nucleosomes were reconstituted as described elsewhere [ 25] using a 208-bp DNA fragment and chicken erythrocyte core histones that had been purified by hydroxylapatite chromatography [ 26].
G418 resistant K562 colonies were counted 2.5 3 weeks after selection and compared to results obtained with pNI ("insulator-less" vector) and pNI-CD (containing 2 copies of the 250 bp chicken b-globin core HS4 insulator).
It's purpose is more than just quirky decor; I think the veggie-chicken captures the core message of Gardner's latest review paper, which appears in Nutrition Reviews.
Indeed, chickens have a core body temperature that ranges from 39 45°C, several degrees higher than that of mammals [25], and xenopus, a poikilotherm, has evolved to adapt to a wide range of external temperatures [26].
Nan Lu, a doctor of traditional Chinese medicine, said the black chicken is at the core of the healing benefits.
The rest of the name is darkly humorous, as it means "mother and child" – referring to the two core ingredients: chicken and egg.
Cannelloni, one evening's special, was indeed that; drizzled with excellent Bolognese sauce and a dollop of bechamel, fresh pasta skin wrapped a harmonious forcemeat core of chicken, beef and spinach.
To determine if we could detect the presence of methylated lysine 37 in higher eukaryotes, we performed Western blot analysis comparing oligonucleosomes isolated from chicken erythrocyte nuclei and core histones from HeLa cell nuclei to yeast histones.
As with the previous report on vitreous samples from chickens [13], multiple HS core proteins were observed.
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