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In a model chicken cell line, we overexpressed a mutant form of MINPP that is secretion-competent.
As a representative case, chicken cell nuclei show highly consistent radial chromatin arrangements: gene-rich, early replicating microchromosomes are clustered within the nuclear interior, while gene-poor, later replicating macrochromosomes are preferentially located at the nuclear periphery.
For this purpose the permanent chicken cell line DF-1 [27] was used.
In agreement with this, our own studies in the chicken cell line DT40 have shown that PCNA ubiquitination is required for post-replicative gap filling, but not for maintenance of fork progression on a damaged DNA template [16].
Using a combination of mitotic figure analysis, cell labeling and long-term cell tracing, we show that, in chicken, cell division does not play a major role during the entire ventral mesoderm differentiation process after gastrulation.
Twelve different cell lines from mammalian and avian species (BHK-21 HEp-2, Vero, HeLa, MDCK, DF-1, MDBK, PK-15, RK-13, CCL-141, QT-35 and BT) and two primary chicken cell cultures (CEF and CEK) were evaluated to identify cell substrates that can support the replication of APMV-5.
Similar(30)
This perhaps imposes some limitations on the repertoire of peptides recognized by chicken T cells.
Mr. Kovacs's most fascinating project involved cultivating embryonic chicken cells.
By the time Hayflick proved this, Carrel was long dead and his "immortal" chicken cells discarded.
Viruses that replicate well in chicken cells differ slightly from ones that replicate well in human cells.
Here we present plasmid and retroviral RNAi vectors specifically designed for optimal gene silencing in chicken cells.
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