Exact(5)
The control group was artificially fed on hamster blood through chick skin membrane.
This suspension was used to feed Lutzomyia longipalpis sand flies through a chick skin membrane.
Three-day-old female sand flies were fed through chick skin membrane on hamster blood containing 107 promastigotes/ml from exponential growth phase culture of L. i. chagasi and flies were dissected at days 3 and 7 post-infection to confirm the presence of parasites [20].
Female flies were infected by membrane feeding through a chick skin with a rabbit blood meal seeded with 2 × 10 ml− 1 amastigotes of Le. infantum or Le.
For the qPCR experiment, females were fed through a chick skin on heat-inactivated rabbit blood containing L. infantum infected macrophages (or uninfected macrophages in the control group).
Similar(51)
Female sand flies were infected by feeding through a chick-skin membrane on heat-inactivated rabbit blood containing promastigotes of each parasite line.
Sand fly females were infected by feeding through a chick-skin membrane on heat-inactivated rabbit blood containing 10 promastigotes ml−1.
Rabbit blood seeded with parasites was offered to 5-day-old Lu. longipalpis through a chick-skin membrane and infected female sand flies collected daily from 0 to 5 days post-feeding.
For time-course experiments, sand flies were fed on chicken and human blood through chick-skin feeders at 35 °C and each group sampled at 24, 48, 72, 96, and 120 h intervals from the day of the feed (day 0).
As Noggin and Sonic hedgehog (Shh) were recently shown to be required for early chick ventral skin formation, and able to trigger skin and feather formation from chick amnion, we associated cells engineered to produce those two factors with mouse amnion.
As a result, 1 × 10 cells were yielded from 1 cm of chick embryo skin.
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