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Water chemistry data were collected and analyzed by the Kansas Department of Health and Environment (KDHE) as part of their stream chemistry monitoring network (KDHE 2000).
Important clinical and laboratory variables, such as age, sex, preexisting underlying disease, clinical diagnosis, severity of infectious condition, microbiology of culture from infection source, hematologic data, and chemistry data, were collected when CRP and PCT tests were conducted.
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Reaction conditions were 95°C for 15 min, 36 cycles of 95°C, 30 s; 58°C 30 s; 72°C, 30 s, followed by 72°C for 10 min. DNA sequencing of PCR products, using primers 5′-GGAGTTTCGCTCTTGTCACC-3′ (rs1137918) and 5′-GTGCAGTGGCATGATCTCAG-3′ (rs227091), was carried out using standard dye terminator chemistry and data were collected using an Applied Biosystems Genetic Analyser 3130xl.
The sensor data were collected while blinded to the clinicians and hourly reference glucose data were collected using a chemistry analyzer (YSI 2300D).
Data were collected prospectively.
Data were collected anonymously.
Data were collected 24/7.
Baseline data were collected.
Demographic data were collected.
Data were collected retrospectively.
Data were collected online.
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