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Hepatic volume (estimated by magnetic resonance imaging [MRI]) and blood chemistries were measured at the same intervals.
Blood chemistries were measured using serum from each individual mouse using the Olympus AU400 automated chemistry analyzer.
Urine and plasma chemistries were measured by the Clinical Chemistry Laboratory of Jilin University, China.
Complete blood counts, blood urea nitrogen (BUN), serum creatinine and comprehensive serum chemistries were measured.
Biochemical determinations: routine chemistries were measured using the Vitros® 4600 Chemistry System.
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Haematological indices and serum chemistry were measured at 84 and 112 d of study.
At both midterm and final assessments, hematological parameters and serum chemistry were measured, and organ weights and histopathology were examined at the end of the trial.
In this study, the anionic charge densities of several polycarboxylates based on methacrylate ester chemistry were measured in aqueous solution at pH 7 and 12.6, resp., and in cement pore solution.
Full blood count and clinical chemistry were measured in plasma and serum samples according to the laboratory standard operating procedures.
Height, weight, body mass index (BMI), body fat mass, systolic blood pressure, diastolic blood pressure and blood chemistry were measured once a year.
Arterial blood gases and chemistry were measured with the Instrumentation Laboratory 1640 blood gas analyser (Lexington, MA, USA) and Synchron LX20 (Beckman Coulter Inc., High Wycombe, Buckinghamshire, UK) The total base deficit (BDtot) was calculated according to the algorithm for 'standard base deficit' in the blood gas analyser, which necessitated the concurrent measurement of haemoglobin.
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