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Sensitive real-time sequence detection methods based on two different chemistries were developed for Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of Johne's disease in cattle.
Because of its prominence, several new Q-PCR detection chemistries were developed, reaching approximately 20 different at present [ 1].
However, as new generation oligomer chemistries were developed and experimental design was refined and became more rigorous, additional methodologies to manipulate gene expression using AOs were developed, including translation suppression, gene silencing and modification of pre-mRNA splicing (for review see Bennett and Swayze 83).
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Sterical improvements on the linker chemistry were developed for high-affinity binding.
A new clusterable RTCVD module for TiN deposition using TiCl4 and NH3 chemistry was developed and built up by STEAG RTP Systems GmbH.
Chemistry was developed to introduce functionality onto the 9-aryl ring, which resulted in the identification of potent FAK inhibitors.
A new method of coupling reactions and mixing processes based on manifold points with detailed chemistry is developed.
To address the problem of detecting inhibition in purified nucleic acids, an exogenous internal positive control (IPC) based on Taqman® chemistry was developed.
Previously a one-tube real-time RT PCR assay based on TaqMan® chemistry was developed and shown to be ideally suited to PSTVd detection.
A real-time RT-PCR method utilizing SYBR Green chemistry was developed to detect and enumerate hepatitis A virus (HAV) in ocean water.
Finally, in the third case, a thiol-based chemistry was developed for long incubation times of biological samples of high complexity.
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