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Here, the most responsible molecular parameters for the pure liquid molar volume of organic and simple inorganic chemicals were determined through a robust quantitative molecular structural analysis.
The protein concentration (Pierce BCA) and pH (ColorpHast, pH 2 9, EMD Chemicals) were determined.
The effects of various metal ions and chemicals were determined under the standard reaction condition.
The HDAC inhibition activities of chemicals were determined by the popular two-step fluorogenic assay method, and the protocols of the QM/MM MD simulations are similar to our previous study of HDAC.
The RC50 values for MMA and a variety of other LMW chemicals were determined by Schultz and colleagues (Schultz et al., 2009; Yarbrough and Schultz, 2007) using a spectrophotometric assay to measure sulfhydryl group depletion following 2-hour reactions with GSH.
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Systemic bioavailability of chemicals was determined by calculating area under the plasma concentration curve over 24 h (AUC-24 h) using complete sets of data (≥5 data points) and also three, two, and one selected time points.
Thus, the interactivity of two chemicals was determined by the last score.
The refractive index for both chemicals was determined using a temperature-controlled refractometer at 23°C (Fisher Scientific; model no. 334620).
The overall absorption and distribution of chemicals are determined by both passive diffusion and mediated transport, and cannot be understood without a proper consideration of both contributions.
The intracellular concentration of chemicals was determined on the basis of the concentration in the pipet and the volume of injection.
Thus, the assessment of risks in response to chronic low-dose exposure to chemicals is determined by examining acute toxic effects of only a few very high doses.
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