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Well-defined peaks were obtained for the four chemicals under study, with retention times of 2.2, 2.8, 4.2 and 6.8 min for MP, EP, PP and BP, respectively.
Furthermore, confounding effects such as autofluorescence of cellular constituents or the chemicals under study and cytotoxicity may complicate the data analysis and interpretation.
After completion of the interview, blood collection equipment determined to be free of the environmental chemicals under study was used to obtain 10 cc of blood.
It should be pointed out that use of the toxic equivalence factor approach (Safe 1998) assumes common dose response slopes across the chemicals under study; the general dose-addition definition of Equation 1 does not require such an assumption.
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Since every spectral feature is due to an interaction of photons of particular energies with the atoms and electrons within the chemical under study, then the nature of the absorption is largely unique to the specific chemical structure where the concept of a diagnostic absorption feature is used for it.
There is also the chance that the particular chemical under study differs in its relative life-stage specific/adult sensitivity ratios from the geometric mean of other chemicalife-stage specific/adult obsensitivity data.
All the reagents, media, and chemicals used under study were of analytical grade purchased from Qualigens Fine Chemicals Pvt Ltd , HiMedia Laboratories Pvt Ltd , Merck & Co.and Loba Chemie Pvt Ltd from Mumbai, Maharashtra, India.
The EVB approach (which has been discussed in detail elsewhere [52,120]) has proven to be a highly useful and efficient approach in a number of studies [28,51,99,118,119,121], as it is fast enough to provide both extensive conformational sampling and provides sufficient chemical information to describe chemical processes under study.
This selection should be made so that the range of the variation of interest to the chemical problem under study is spanned by the selected items.
The goal of this research work is to develop a useful and universal estimation procedure to determine simultaneously the kinetic parameters involved in the chemical modelling under study such as combustion, pyrolysis, waste stabilization, etc.
Even though DFT has been shown to be highly efficient compared to other ab initio approaches (see discussion in Section 2), it has also been shown to suffer a number of limitations [124 126] which can be more or less critical depending on the particular characteristics of the chemical process under study.
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