Sentence examples for chemically defined medium for from inspiring English sources
In this study, a serum-free, stroma-free, and chemically defined medium for hematopoietic stem cell (HSC) expansion was systematically developed and optimized using factorial design and the steepest ascent method.
These cell lines can be easily adapted to serum-free suspension culture by gradually exchanging the medium to a serum-free, chemically defined medium for suspension cells (data not shown).
Streptomyces sp. 235 was able to secrete polypeptides to the extracellular environment during its growth in a chemically defined medium for 7 days, as demonstrated by SDS-PAGE analysis).
For the experiments described in Figure 3, enriched astrocyte cultures were prepared using the shaking method and then transferred to a chemically defined medium for 5 days to enhance their maturation (S.W. Levison, unpublished data).
CHO cells adapted to serum-free suspension culture (CHO-SF, Invitrogen, Grand Island, NY), were grown in protein free chemically defined medium for CHO cells (CD-CHO medInvitrogenrogen) supplemented with 1× HT supplement (Invitrogen), 4 mM glutamine and 50 μg/ml dextran sulfate (MW: 500 000, Amersham Pharmacia Biotech, Uppsala, Sweden).
Human ES cell line H9 (from WiCell Research Insititute. Simple Letter Agreement: #10-W0353 to Yijia Lou) was differentiated into INS+ cells according to the protocol of Jiang et al. Human ES cells were plated into 1% Matrigel (BD Biosciences, San Jose, CA, USA -coated dishes and cUSA -coatedh chemically dishesd medium for 2 dand.
To our knowledge, the best chemically defined medium reported for the culture of rat embryos is mR1ECM [34], a phosphate free media used in these studies.
Adaptation of continuous cell lines to growth in suspension in a chemically defined medium has significant advantages for design and optimization in manufacturing of biologicals.
We used here a simple screen on chemically defined medium (CDM) to search for potentially new nutritional requirements of F. tularensis.
To further underscore the genomic stability of our tetraploids in exponential phase, we grew a pool of strains in chemostat continuous culture in a complex, but chemically defined, medium [ 30] for a period of 4 days (30 to 35 generations) and observed no reduction in DNA content (data not shown).
In vitro chondrogenesis was tested in micromass culture in the presence of transforming growth factor beta in a chemically defined medium and assessed by histochemistry for cartilage proteoglycans and by quantitative RT-PCR for chondrocyte markers.
