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These slices were either dropped into chemical fixative or cryo fixed, after they had first been allowed to recover in medium for 40 min at 30°C.
The stress, here generated by having to perform in front of others, has a role like a chemical fixative in developing a photo: it fixes in the brain the content of what is explained.
Under Dr. Mikula's method, called chemopreservation, neuroscientists first insert a needle filled with a chemical fixative into an anesthetized animal's heart while it is still alive to pump the fixative through the brain, essentially gluing its structure in place.
Half the bacteria were then mixed with more growth medium to keep them alive until they returned to Earth; the other half were mixed with a chemical fixative that stopped their growth and preserved them so that their gene expression could be studied after the shuttle landed.
However, most of the endogenous oxidases should have lower tolerance to strong chemical fixative than APEX/APEX2, which is the prerequisite to ensure APEX/APEX2 can work without interruption from endogenous oxidases.
After preserving the brain in formalin, a chemical fixative, and embedding it in wax for several months, they began to cut it using a knife called a microtome, which carves linked sections onto a conveyer belt like a deli slicer cutting turkey breast.
Similar(50)
As cells are examined in the absence of any chemical fixatives, stains, or contrast enhancement reagents, the images reflect cellular structures in the near-native state.
While universally employed for the preservation of cell organization, chemical fixatives often introduce artifacts that can confound identification of true structures.
The HPF approach offers to preserve organelles and other cellular components by instantaneous stabilization [43] in a manner superior to chemical fixatives [44].
The above methods rely on an initial fixation step, usually using chemical fixatives.
One approach, designed to improve the safety of self-antigen delivery in AID, involves the coupling of intact antigen to APC using chemical fixatives [ 36].
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CEO of Professional Science Editing for Scientists @ prosciediting.com