Sentence examples for chelate tags from inspiring English sources

Techniques for labeling biomolecules with lanthanide chelate tags are discussed, with aspects of chelate design.

Using time-gated acquisition the background signal can be efficiently suppressed, enabling more sensitive detection of proteins using lanthanide chelate tags than is possible with chromophore-based or conventional fluorophore-based labels.

We compared several purification methods based on affinity chromatography for purification of proteins from cell culture supernatants: metal chelate affinity, strep-tag and immunopurification with a monoclonal antibody.

There are also some examples using His in the target areas of the protein and using supports modified with immobilized metal chelates and other tags (e.g., using immobilized antibodies).

Talon beads, which employ Cobalt to chelate the His-tag, were used for the affinity purification as trial experiments proved them better than Ni-based beads (data not shown).

All NTRs, transport substrates and OGT were purified by native Ni II) chelate chromatography, Tev-cleavable tags were cleaved with Tev protease (with exception of His10-GFP-Tev-Impα). Proteins were further purified by gel filtration on a Superdex 200 column equilibrated in 50 mM Tris HCl pH 7.5, 200 mM NaCl, 2 mM DTT, and snap-frozen after adding 250 mM sucrose.

(6) His-Tag Chelating Strategy on Liposome Surface.

It turned out, unfortunately, that 99mTc is also chelated by HEHEHE-tag, and the site specificity of labeling is lost (Lindberg et al. 2012).

In addition, enzymatic ligation of proteins to preformed liposomes and the His-tag chelating strategy for modifying the liposome surface will be described.

The protein has an N-terminal hexahistidine tag for nickel chelate affinity purification, the D424A mutation to inactivate the 3′ 5′ exonuclease, the C907S mutation to remove the single native cysteine, and the L744C mutation to provide a unique labeling site on the fingers subdomain.

Sequences and plasmid maps are available on request.> All NTRs, transport substrates and inert molecules were purified by virtue of N-terminal His-tags and native Ni II) chelate chromatography.