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A strongly scented variety, Basmati370 and a non-scented variety Jaya were used as checks for scoring of aroma, Alkali Spreading Value (ASV /Gelatinization temperature (GT) - The method of Little et al. [ 21] was used for conducting the alkali spreading test.
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Markers that deviated significantly from Mendelian ratios were re-checked for scoring errors.
Genotypes were checked for scoring errors using MICRO-CHECKER v. 2.3 [ 93].
Resulting genotypes were checked for scoring errors and potential null alleles using Micro-Checker [ 72].
The microsatellite data set was checked for scoring errors due to stuttering, large allele dropouts, and null alleles with the program Microchecker [ 27].
MICRO-CHECKER v.2.2.3 (van Oosterhout et al. 2004) was used to check for scoring errors caused by stutters or large-allele dropouts and to estimate null-allele frequencies.
Genotypes were checked for scoring errors because of null alleles, stutter and large allele dropout using Microchecker 2.2.3 (van Oosterhout et al. 2004).
Genotypes were checked for scoring errors that might be attributable to stutter-products, large allele dropout or to the presence of null-alleles, using the software micro-checker 2.3 (Van Oosterhout et al. 2004).
We used MicroChecker (Van Oosterhout et al. 2004) to check for scoring errors per population, which could result from stuttering, large allele dropouts, and null alleles (Dewoody et al. 2006).
Micro-Checker v. 2.2.3 [ 23, 24] was used to check microsatellite data for scoring errors and null alleles.
The agency also has launched a survey to check for scores of drugs at water treatment plants across the nation," the AP says.
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