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GalK deletion mutant BACs were also checked for absence of undeleted contaminants by PCR across the deleted locus.
Melt curves were analysed to check for absence of mispriming.
Cell lines were checked for absence of mycoplasm contamination (MycoAlert mycoplasma detection kit, Lonza, Swizerland).
Supernatants were harvested 48 and 72 hours after transfection and were checked for absence of replication-competent virus.
Non-diseased control tissues (n = 5) were taken from biopsy and autopsy material that was checked for absence of pathology.
Supernatants were harvested 48 and 72 hours after transfection and checked for absence of replication-competent virus.
The loci used in this study were previously checked for absence of intragenic recombination (Druzhinina et al., 2008).
Non reverse-transcribed RNA samples were checked for absence of contaminating genomic DNA by PCR using primers for the constitutively expressed elongation factor alpha-chain gene (At5g60390).
Isolated mutants were checked for absence of the 1.3-kb plasmid sized, intron I containing, ermB gene using primers ErmRAM-F and ErmRAM-R.
The resulting monokaryotic cultures were microscopically checked for absence of clamp connections and 23 progenies derived from Lmsa110092 were included in this study.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com