Table 4 stores modified record ids and time stamp of recording the checkpoint in a table called checkpoint table.
CKP_F 1 —– F 2 —– T 3 —– T 4 —– F 5 —– F Table 4 Checkpoint table CKP_id TS (Time Stamp) R_id 1 1.0 2,3 2 1.1 1,3 3 1.2 0. For the various classification of nodes the specific failure handling strategy in case of node failure is given.
The dephosphorylation of CDC25, the expression of cyclin E, the degradation of the cyclin E-CDK2 complex, and the concentration of the transcription factor E2F were classified as the most fragile reaction steps in the G1/S checkpoint (Table 1, cluster I).
Categories in which the proportion of IR-responsive genes exceeded expectation based on chance included cell cycle, cell proliferation, DNA and RNA metabolism, M phase of mitotic cell cycle, S phase of mitotic cell cycle, response to DNA damage stimulus, DNA repair, and cell-cycle checkpoint (Table 2).
Others, including P21-P53_Middle_DN, UBV_NHEK1_C2, and TGFBPATHWAY, emphasize anticancer and cell cycle checkpoints regulation (Table 2).
Still, they can be genetically distinguished by the mediator protein that connects the two major sensors (Mec1/Rad3/ATR and Tel1/ATM) with the two major effectors (Rad53/Cds1/Chk2 and Chk1) of both pathways: Rad9/Crb2/53BP1 is present in the DNA damage checkpoint, and Mrc1/Claspin in the DNA replication checkpoint 84– 88 (Table 1).
The secondary signal is provided through one of several checkpoint receptors (Table 10), which can either provide a costimulatory signal to activate the T cells, or a blocking signal to dampen T cell response (Topalian et al., 2015).
pombe checkpoint genes (Table 2).
The test leader used strict checkpoints (see Tables 2, 3 and 4) for assessment of all learning objectives and criteria.
Therefore, in our current study, we stably introduced four LS-associated missense mutations into the MSH2-null human endometrial cancer cell line Hec59 to determine their effects on in vivo cellular functions such as DNA repair and checkpoint response (Table 1).
