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The mutants were also tested for Chk1 checkpoint function as measured by the ability to survive exposure to DNA damage generated by UV light.
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The apparent checkpoint proficiency of BubR1Δ432 484 may indicate that BubR1 localization to kinetochores is not essential for checkpoint function, as it implies that substantial amounts of MCC can be generated even when BubR1/Bub3 cannot be recruited to kinetochores.
We compare the obsolescence function as measured by readership with the obsolescence function as measured by citations.
This observation suggests that nonphosphorylatable Xlf1 causes a deceleration of the checkpoint response, though full induction of the G2 checkpoint, as measured by Chk1 phosphorylation, is still achieved within 30 min.
Ptc2 and Ptc3 were required for recovery from the checkpoint as measured by Rad53 dephosphorylation and release from the G2/M checkpoint [ 120, 121].
The tagged receptor functions normally as measured by the ability to activate a GIRK conductance.
That instrument, Isaacs says, would have served some unique functions, such as measuring soil moisture.
These cross-cutting symptom measures target more general mental function as well as severity measures that are disorder specific.
Thus, use of a heterozygous dbf4-ΔN221/DBF4 strallowedowed us to separate the checkpoint function of DBF4from its replication function (as well as other meiotic functions, which appear unaffected in the dbf4-ΔN221/DBF4 strain).
To examine whether the replication checkpoint is required for scaling the replication profile with S phase duration we measured the genome-wide replication profile of different MRC1 alleles that separate its checkpoint function from its role in normal replication, and further analyzed the replication profiles of S phase mutants that are checkpoint deficient.
As expected, CHK1 inhibition also diminished G2/M checkpoint function (Fig. 4C and Supplementary Figure S3).
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