The functional recovery of the checkpoint function of CHFR was evaluated also in HCT116 cells after the exogenous introduction of wild type and mutant CHFR proteins.
This suggests that there is a functional link between the anti-proliferative effects and checkpoint function of CHFR.
Spindle checkpoint function and cellular sensitivity to antimitotic drugs.
As expected, CHK1 inhibition also diminished G2/M checkpoint function (Fig. 4C and Supplementary Figure S3).
Takai, H. et al. Aberrant cell cycle checkpoint function and early embryonic death in Chk1−/− mice.
Lu, R., Niida, H. & Nakanishi, M. hSAD1 kinase is involved in UV-induced DNA damage checkpoint function.
Arlt, M. F. et al. BRCA1 is required for common-fragile-site stability via its G2/M checkpoint function.
Brady, D. M. & Hardwick, K. G. Complex formation between Mad1p, Bub1p and Bub3p is crucial for spindle checkpoint function.
BRCA1 is required for HR-mediated DSB repair at Flex1, and its checkpoint function is also needed for CFS protection49.
Kalitsis, P., Earle, E., Fowler, K. J. & Choo, K. H. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis.
Such a detailed model may prove useful for predicting DNA damage G2 checkpoint function in cancer and, therefore, sensitivity to cancer therapy.
