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These stages range from template preparation, nucleic acid quality, reverse transcription (RT) strategy up to the PCR conditions and data reporting [ 20], and checking validation parameters and assay performances following the recommendations of the Minimum Information for publication of Quantitative real-time PCR Experiments (MIQE) guidelines [ 21].
Bonate defines the steps for model development as problem analysis, experimental conduct, data collection and cleaning, model formulation, model fitting, model checking, validation, interpretation, and communication (with iterations).
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This paper will present a comprehensive set of numerical simulation results, using ABAQUS/Explicit, to check validation of the simplified analytical method for the various energy terms and the final result of column critical velocity.
We measured DNA concentrations of 15 hordein clones as described previously in DNA, RNA and cDNA section and carried out the qRT-PCR reactions with selected primers to check validation of the system.
The work involves design considerations, design checks, validation and sensitivity analysis to achieve the design criteria to fulfill the structural requirements for mechanical integrity.
The cross-check validation fortified the acceptance of TLR2-ECD, TLR2-TIR, and MyD88-TIR models.
The cross-check validation report indicated acceptability between the experimental structures (PDB ID: 2Z81, 1O77, and 2Z5V) and their models.
For further validation regarding accuracy of homology model and methods used to generate the three-dimensional model, a cross-check validation approach was used.
Cross-check validation was carried out using model as template and the primary amino acids of the respective template as target.
In this paper, we detail each stage of the experimental protocol, check validation parameters and report assay performances, following MIQE guidelines.
The strengths of the design included the data transcription by the interviewer, analyses by two researchers, a search for disproving data, and member check (validation of the findings by the participants) [ 11, 14, 16].
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