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That is, first compute the set S of all frequent homomorphic patterns, and then do the maximality check and eliminate non-maximal patterns by checking the specificity relation on every pair of patterns in S.
In addition, the PCR products were validated by checking the specificity and size on 1% agarose gel.
When checking the specificity of MON 810 assays, no cross-reactivity was observed with a mixed sample containing 8 different GM maize lines (Table 4).
For checking the specificity of primers towards coral or algal cDNA, a standard PCR amplification was carried out using aposymbiotic coral cDNA (from the coral A. millepora gametes) and coral-algal cDNA mix (from A. millepora).
The Wantai anti-HEV IgG ELISA uses a peptide encoded by a structural region of ORF-2 of HEV genotype 4. Specificity is difficult to assess in situations other than acute hepatitis E because there is no gold standard for checking the specificity of the current anti-HEV ELISA.
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To check the specificity of linkage-specific anti-ubiquitin antibodies, Lys 48- or Lys 63-linked polyubiquitin chain (Enzo Life Sciences) was used as a positive control and subjected to immunoblotting.
We designed human WNT10A and mouse Wnt10a specific primers for semi-quantitative RT-PCR analysis and checked the specificity of the primers (Figures S2A and Table S2).
After PCR amplification, a melting curve was generated to check the specificity of each PCR reaction (absence of primer dimers and other non-specific amplification products).
Moreover, MRM spectra of blanks with mass fragmentation patterns of CVA and IS were obtained to check the specificity of the method.
Eighty four other bacterial strains, either commonly occurring in sheep or found in the environment of sheep, and including organisms related taxonomically to D. nodosus, were used to check the specificity of these assays.
We checked the specificity for different 17β-HSD types and species.
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