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Further, checking the expression levels of N-protein, CCR1 as well as miR-223 in an infected BASC will provide a more compact conclusion.
We also monitored potential toxic and side effects of siRNA experiments, such as interferon response, by checking the expression levels of four genes (p53, IFITM1, Oas2 and Mx1) (Fig. 2A).
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Because miR-487b-5p miR-487b-5p miR-487b-5pp did not express in human tissue (miRBase 19), we checked the expression levels of the rest six miRNandin miR-467a-3p miR-467a-3p miR-467a-3p.
The primers qRT-1 F and qRT-1R were used to check the expression levels of OsHsp18.0-CI in the OsHsp18.0-CI-OE lines, and for the OsHsp18.0-CI -RNAi plants, the primers qRT-2 F and qRT-2R were used.
We further checked the expression levels of CCR6 in an EAE-relevant T cell system.
In order to test this hypothesis we checked the expression levels of PTEN by Western blot analysis, and found elevated levels of PTEN in tumor samples (figure 5e).
We then checked the expression levels of PGC1α, Ucp1, Cidea and mitochondrial proteins in differentiated Fsp27−/− MEFs in the presence or absence of T3.
To determine the extent of shifting of the physiology and gene expression profiles of WAT to BAT in Fsp27−/− mice, we checked the expression levels of BAT-specific genes and several transcriptional regulators by real-time PCR analysis.
In order to check the expression levels of the TAP-ISG15 proteins compared to the endogenous ISG15 levels, the different stable cell-lines were stimulated for 26 h with IFNβ (Peprotech) prior to Western blot analysis.
We subsequently checked the expression levels of the identified danshen genes in different tissues.
Finally, we also checked the expression levels of both alleles by qRT-PCR.
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