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Vectors for checking the expression of constructs in mammalian cells and for harboring the foreign genes as DNA vaccine are suggested.
The confirmation of transfection was examined by checking the expression of EDA in control (non vector), vector control and EDA vector transfected T47D cells by reverse transcription-PCR and western blotting (Fig. 6a,b).
Further, checking the expression levels of N-protein, CCR1 as well as miR-223 in an infected BASC will provide a more compact conclusion.
We have also excluded that any aberrant event could account for the presence of nestin+/GFAP+ cells during differentiation at 1% oxygen, by checking the expression of early markers specific for neuronal and astroglial lineages.
However, we got different results when checking the expression of c-Myb protein.
The polarization was verified by checking the expression of polarization marker genes for Th1 and Th2 subsets.
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Diane studies her sons through the lenses, looking for body language that might suggest an injury, checking the expressions on their faces.
"I think that we should be eternally vigilant," he added, "against attempts to check the expression of opinions that we loathe and believe to be fraught with death".
As Justice Holmes once said, "we should be eternally vigilant against attempts to check the expression of opinions that we loathe".
I'm with Justice Oliver Wendell Holmes Jr., who wrote in 1919 that: "I think we should be eternally vigilant against attempts to check the expression of opinions that we loathe and believe to be fraught with death".
In addition, we checked the expression of the MRs in the Genotype-Tissue Expression (GTEx) Project portal to see if our genes of interest are expressed in stress-related regions of the brain.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com