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Checking the assembly ACE file, we found that no read can bridge the two neighboring contigs long enough for them to be connected by assembler.
Finally, the panel proved useful for checking the assembly of sequence scaffolds and for mapping EST located on one of the smallest microchromosomes.
In addition to the expected advances in fine-mapping of Quantitative Trait Loci (QTL) [ 1, 2], high-density linkage maps provide a framework for checking the assembly of genome sequences and for studies of the evolution of these genomes through the analysis of recombination.
In addition to their use in checking the assembly of genome sequences, high-density maps can help in understanding the evolution of understudied genomes by analysis of recombination (Tortereau et al. 2012; Van Elferink et al. 2010; Vingborg et al. 2009; Wong et al. 2010).
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We checked the assembly for completeness against single-copy, conserved eukaryotic genes using the core set in CEGMA and the vertebrate set in BUSCO, using the transcriptome option.
He checked the assembly line, met with his board and made certain all the American flags were in place and the ribs and catfish ready to barbecue.
The field hand-over test of equipment is required to check the assembly quality of equipment following field installation and before being put into operation to evaluate the equipment performance.
To check the assembly result, nine partially overlapped fragments covering the entire C. oliveri cp genome were amplified and sequenced.
The PCR to check the assembly was conducted using the TaKaRa Ex Taq system and the primer pairs used are listed in Table 1.
Check the assembly at this point to make sure it is square.
If only some of the keys do not perform correctly, check the assembly near them in particular.
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