Drexler and Uphoff [ 2] suggest using at least two detection techniques when checking samples for Mycoplasma.
(g) Check sample: Check sample by re-runing mid point calibration curve in middle and at end of sample sequence.
Fish Canyon Tuff zircons (Gleadow et al. 2015) were also run as "unknowns" with each batch of samples to check sample accuracy.
As such it is important to check sample veracity prior to analysis.
Should there have been a difference in opinions of more than one fifth of the doubly checked sample, further checks would have been completed.
For 94 subjects, there was adequate sample to prepare and analyse at least two replicate subsamples to check sample homogeneity and analytical reproducibility.
For all datasets, the program Graphical Representation of Relationships (GRR) [ 20] was used to check sample relatedness and to correct potential sample mixups, duplications, or contaminations.
Using SPSS version 19 (IBM-SPSS Inc., 2010, Chicago, IL, USA), descriptive analyses were run in order to check sample and symptom characteristics.
In addition, tags were also mapped against a database of 8,662,308 CDS and 5,189 genomic sequences from bacteria directly downloaded from GenBank [ 110] repositories to check sample contaminations.
To double-check samples of these animals' urine will also be analyzed at the board's laboratory in Jamaica.
