For temperature-sensitivity check, 10-fold serial dilutions of cultures were spotted on L plates and the plates were incubated at 30 or 37°C for 24 h.
At the very least, it's an annual reality check that there is a world of cultures other than our own, and some of them are quite adept at writing pop songs.
The purity of cultures was periodically checked using immunofluorescence, and the cells were negative for human factor VIII antigens and cytokeratin and positive for actin and Thy 1. Confluent MCs were resuspended in 2.5 ml Tyrode buffer containing 0.2% bovine serum albumin and incubated in a CO2 incubator at 37°C for 30 minutes.
For a lighter dose of culture, check out the temporary exhibition "Between Sculpture and Fashion," which runs through Oct. 30.
To avoid a pH effect, when SCFA were added in culture medium, the pH value was checked and adjusted to those of culture medium.
From the 45 cultures analysed, 33 were checked after only 6 hours of culture and none showed clumps of GFP+ cells (Fig. 7D), again suggesting that this segregation is an active process.
Americans enjoy the notion that our commitment to transparency and accountability is a self-correcting constraint against the sort of clannish looting that infects authoritarian systems; Chinese officials prefer to imagine that the Party's devotion to sacrifice and fairness checks the culture of greed and self-dealing that infects capitalist political culture.
If the logos on the sitters' shirts — YouTube, Izod, Nike — seem to refer to the dissemination of culture through commerce, check out the gift shop, where Wiley's images grace beach towels, Puma shoelaces, and dog tags.
In order to check that after a first step of culture under hypoxia, the differentiation into SMCs was stable versus time, cells were further cultivated either under hypoxia or normoxia.
