However, Asian bat lyssaviruses (1, 2, 4 ) were unavailable at that time to check for cross-reactivity.
Control sections (no primary antibody) were included to check for cross-reactivity and bleed through of fluorescence.
If these molecules are antigenic they would then need to be checked for cross-reactivity by antibody recognition.
Both the primary and secondary antibody were incubated with cells alone to check for cross reactivity.
As the technology is an array, all components were checked for cross reactivity with other components in the antigen and antibody cocktails and confirmed to have less than 0.5% cross reactivity.
Additionally, to check for possible cross-reactivity of the HBDS-ELISA with a view to confirming its specificity, positive sera of classic swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine pseudorabies virus (PRV), and porcine parvovirus (PPV) from PCV-free pigs were tested according to the HBDS-ELISA procedure.
A multitude of software tools have been developed implementing algorithms for the design of general mammal-specific, and virus-specific siRNAs that are automatically checked for possible cross-reactivity.
The designed probes were checked for melting temperature, secondary structure, and GC content by using the Vector NTI Advance 9 (Invitrogen, Carlsbad, California, USA), and checked for potential cross-reactivity with other species in GenBank by using the BLASTN program.
To control for cross-reactivity in double immunofluorescence, sections were incubated with secondary antibody only.
Unsuitable regions are filtered out before checking for cross-hybridization.
