checking for cross-hybridization

USAGE SUMMARY

The phrase "checking for cross-hybridization" is correct and usable in written English.
It can be used in scientific or research contexts, particularly in molecular biology or genetics, when discussing the process of verifying that a probe or sequence does not bind to unintended targets. Example: "Before proceeding with the experiment, we will be checking for cross-hybridization to ensure the accuracy of our results."

✓ Grammatically correct

Science

Human-verified examples from authoritative sources

Exact Expressions

1 human-written examples

Unsuitable regions are filtered out before checking for cross-hybridization.

BMC Genomics

Human-verified similar examples from authoritative sources

Similar Expressions

59 human-written examples

All primers and probes were checked for possible cross-hybridization with bacterial genes using the database similarity search program BLAST.

BMC Oral Health

All selected primer sequences were then checked for possible cross-hybridization using the BLAST [ 43] and subjected to quality check using Net Primer [ 44].

BMC Veterinary Research

Multiple antibody labeling was done simultaneously after checking for cross-reactivity.

ACS Nano

Finally, the oligonucleotide was checked for Secondary Structure Folding Tm and Maximum Cross-Hybridization Tm using the Tethys oligonucleotide design backend, taking note of the potential for cross-hybridization.

BMC Genomics

In a breeding program, usually only superior parents are chosen for cross-hybridization (Ajeeshkumar and Decruse 2013).

Botanical Studies

Probesets with an annotation of a high potential for cross-hybridization were also removed.

Plosone

These probes were further verified computationally for cross-hybridization.

BMC Medical Genomics

We developed the following strategy to correct for cross-hybridization.

Bioinformatics

Any significant cross-hybridization was eliminated, where possible, or noted, thereby minimizing the potential for cross-hybridization with other genes, which would generate false positive results.

BMC Genomics

Each probe was also assessed for cross-hybridization within the same genome.

BMC Genomics

Expert writing Tips

Best practice

When designing primers or probes for molecular biology experiments, always include a step for "checking for cross-hybridization" against relevant databases to ensure specificity and avoid false positives.

Common error

A common mistake is to perform "checking for cross-hybridization" using outdated sequence databases. Regularly update the databases used for sequence alignment to ensure accurate results and avoid spurious cross-hybridization.

Linguistic Context

The phrase "checking for cross-hybridization" functions as a gerund phrase acting as a noun, often serving as the subject or object of a sentence. This reflects an action or process, as demonstrated by the Ludwig examples, related to ensuring specificity in molecular biology techniques.

Expression frequency: Common

Frequent in

Science

100%

Less common in

News & Media

0%

Formal & Business

0%

Academia

0%

Ludwig's WRAP-UP

In summary, "checking for cross-hybridization" is a grammatically sound phrase, acting as a noun phrase within sentences, commonly used in formal and scientific contexts. As Ludwig AI confirms, the phrase's primary purpose is to underscore the importance of verifying the specificity of oligonucleotides in molecular biology. The frequency is common, demonstrating its consistent use in scientific literature. To enhance accuracy, it's important to regularly update sequence databases and consider alternatives like "assessing cross-hybridization potential" for nuanced communication.

More alternative expressions

Phrases that express similar concepts, ordered by semantic similarity:

assessing cross-hybridization potential

Emphasizes evaluation of likelihood rather than the act of checking.

verifying absence of cross-hybridization

Focuses on confirming the non-existence of cross-hybridization.

evaluating cross-hybridization risks

Highlights the risk assessment aspect of cross-hybridization.

validating cross-hybridization specificity

Concentrates on confirming the specificity to avoid cross-hybridization.

confirming lack of cross-reactivity

Similar to verifying absence, but uses the term cross-reactivity.

screening for off-target binding

Broader term encompassing unintended binding, including cross-hybridization.

testing for cross-hybridization events

Focuses on the occurrence of cross-hybridization as events.

analyzing for non-specific binding

More general term for unintended binding, relevant but not specific.

investigating cross-hybridization interference

Highlights the interference caused by cross-hybridization.

ruling out cross-hybridization effects

Emphasizes the elimination of cross-hybridization as a cause.

FAQs

How can I use "checking for cross-hybridization" in a sentence?

You can use "checking for cross-hybridization" in a sentence like: "Before using these primers, we are /s/checking+for+cross-hybridization target="_blank" rel="nofollow">checking for cross-hybridization to avoid non-specific amplification."

What is an alternative to "checking for cross-hybridization"?

Alternatives include "assessing cross-hybridization potential", "verifying absence of cross-hybridization", or "evaluating cross-hybridization risks" depending on the specific context.

Why is "checking for cross-hybridization" important in PCR primer design?

It's crucial because it ensures that the primers will only bind to the intended target sequence, preventing amplification of unintended DNA fragments and reducing the risk of false positive results. Neglecting this step can lead to inaccurate experimental conclusions.

What tools are commonly used for "checking for cross-hybridization"?

BLAST (Basic Local Alignment Search Tool) and other sequence alignment algorithms are frequently used to "assess cross-hybridization potential" by comparing primer or probe sequences against comprehensive sequence databases.