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To this end, we checked the levels of Securin in PGCs by in situ immunostaining with Securin, Mvh and DNA.
We checked the levels of p53 in both U251 and U87 treated cells and observed a significant increase of p53 protein levels in the miR-181c treated U251 cells (Figure6C,D); however we could not detect p53 in the U87 cells.
To further confirm our results from the protein antibody array, we checked the levels of ANG (Figs. 4A and 4D), Ang-1 (Figs. 4B and 4E), and VEGF (Figs. 4C and 4F) in tumor-conditioned medium, endothelial cell-conditioned medium and in conditioned medium from co-cultures using ELISA in cells that were untransfected, transfected with scrambled vector, puPA, puPAR or pU2.
In order to confirm this observation we checked the levels of cell cycle proteins.
We also checked the levels of BECN1, a component of the autophagosome initiation complex.
First, we checked the levels of LET-60 protein in the germlines by immunostaining.
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She also checked the level of CPK -- an enzyme that rises when muscle is injured.
Hence, we checked the level of IL-4 secreted by the lymphocytes following vaccination.
We first checked the level of constitutive activation of the RIG-I constructs in absence of any stimulus in HEK 293T or BEAS-2B cells.
To elucidate the anti-IBD activity of GSC, we checked the level of matrix metalloproteinases (MMPs) and inflammatory mediators.
We also checked the level of ISG expression in response to IFNα in two different clones of hST2-U3C with varying STAT2 mRNA levels.
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