We checked the expression of miR-638 in 64 paired of GC tissues and NCTs, and observed significantly down-regulation of miR-638 in GC, which was in consistent with a previous report (Shrestha et al., 2014).
First, we checked the expression of CTLA-4 in HLN and spleen.
We checked the expression of Sei1 and Sertad2 in a variety of organs by qRT-PCR.
We checked the expression of synaptic markers in 5 week old mice exposed to EE for 14 days.
To confirm these results, we checked the expression of PTEN, Akt and PI3K at both the transcriptional and translational levels.
Then, we checked the expression of TrkA in fresh NK cells from mouse bone marrow, blood and lung.
We checked the expression of Sox-2 at the protein level and found no expression in B10L cells (Figure S1C).
We also checked the expression of the 25-kDa ThTPase by determination of its enzyme activity, immunoblotting and RT-PCR.
For this purpose, we checked the expression of TGF-β receptor-1 and -2 and phospho-smad 2 and 3 in 10A-vec and 10A-TG2 cells.
We also checked the expression of both basal and suprabasal layers markers of the epidermis, namely the keratin 14 (K14) and 10 (K10).
To determine if LR asymmetry of the visceral organs was affected in dmrt2b knockdown embryos, we checked the expression of foxa3 which marks the liver.
