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Because miR-487b-5p miR-487b-5p miR-487b-5pp did not express in human tissue (miRBase 19), we checked the expression levels of the rest six miRNandin miR-467a-3p miR-467a-3p miR-467a-3p.
We further checked the expression levels of CCR6 in an EAE-relevant T cell system.
In order to test this hypothesis we checked the expression levels of PTEN by Western blot analysis, and found elevated levels of PTEN in tumor samples (figure 5e).
We then checked the expression levels of PGC1α, Ucp1, Cidea and mitochondrial proteins in differentiated Fsp27−/− MEFs in the presence or absence of T3.
To determine the extent of shifting of the physiology and gene expression profiles of WAT to BAT in Fsp27−/− mice, we checked the expression levels of BAT-specific genes and several transcriptional regulators by real-time PCR analysis.
Finally, we also checked the expression levels of both alleles by qRT-PCR.
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We then checked the expression level of cell-cycle related genes in R498 and 08sg2.
Notably we checked the expression level of Am1 itself.
Finally, we checked the expression level of the Drosophila ortholog spaetzle, which is a member of the Toll immune signaling pathway against fungi and bacteria [ 42].
To examine whether BRs are required during callus establishment, we checked the expression level of the DWF4 promoter-GUS reporter gene [ 49].
Furthermore, we checked the expression level of the genes CDKN2A and MKI67, biomarkers indicative of an abrogated response to senescence-inducing stimulus [ 33].
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