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We also checked the experiment with negative controls, i.e. absence of primary antibody (Fig 2NC).
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In order to check the experiment used for microarray analysis for effective LPS stimulation and for the expected differences between Wt and Tyk2-/ cells, IFNβ mRNA expression was monitored with RT-qPCR (data not shown).
The location of the coil on the participant's head was visually checked throughout the experiment to ensure the location and angle of stimulation remained unchanged.
The presented methods were checked by the experiment and analytically.
The amount of gas in the balloon was periodically checked throughout the experiment.
For the experiments, light-fluxes of 30 or 5 μmol m−2 s−1 were used, the light-flux was checked before the experiment at the level of the yeast cells (distance yeast cells - LEDs: 10 cm) with an optometer (P2000, equipped with light-detector PD-9306-2, Gigahertz-Optik, Türkenfeld, Germany).
Check the Social Experiment tour dates to catch Rashad, Spinn, and Chance the Rapper in your town.
JG performed the experiments and checked the figures.
CJ guided the idea and the experiments, and checked the figures.
To verify the relevance of our in vitro experiments, we checked the expression of genes in the "Fibroblast specific BMP2 induced gene list" (Additional file 7: Table S7) in publicly available microarray data from lung cancer biopsies [ 13].
Technicians were using a method known as atomic force microscopy to check on the experiment, and in doing so unintentionally captured the first images of atomic bond.
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