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At first, we checked the absence of initial differences in the expression of several gene transcripts involved in the development of morphine dependence in Per2Brdm1 mutant mice and in their respective wild-type (WT) control littermates.
We added sera from 5 mice before injection as negative control and we checked the absence of pI interference in the neutralizing assay using sera from mice injected with pI alone at 200 µg/mouse.
For species where a loss was inferred we manually checked the absence of the particular gene product by using a reciprocal best hit approach against the NCBI non redundant database (nrdb).
Before the apnea test, the physician checked the absence of confounding variables known to interfere with brain-death diagnosis (low arterial blood pressure, hypothermia, neuromuscular relaxation, and circulating psychoactive drugs).
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A 15-s end-inspiratory pause was performed to check the absence of air leak in each experimental condition.
Care must be taken to check the absence of tissue between the organ and the device (bowel, omentum, or fringes of the fallopian tubes) when applying the suction cup.
After checking the absence of jammer, a first test involving non-modulated signals has been carried out to adjust each transmitted power (50 W for channel #1 and 200 W for channel #2) in order to observe balanced received levels.
This step also allowed to check the absence of these cas genes in other locations along the Escherichia/Salmonella chromosomes and plasmids.
This was followed by the measurement of fluorescence during a 55 to 95°C melting curve in order to detect a single gene-specific peak and to check the absence of primer dimer peaks.
FACS analysis was also carried out on HeLa cells treated with buffer in which the erythro-HA-virosomes were resuspended to check the absence of unincorporated 5-Aza-2-dC drug (control-2).
This noninvasive method could be used to check the absence of raised ICP.
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