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During the gradual-salinity-decrease experiment each specimen was checked for survival intermittently 19 times in total.
The researchers checked for survival, discharge from hospitals and more than 200 "unique observations" about each cardiac arrest, its location and the use of CPR and AEDs.
Worms were washed three times in M9 buffer, placed in 1% SDS for 15 min [19], and then checked for survival.
Flies were daily checked for survival until natural death or experimental checkpoint (21 days).
If the growth is arrested at the concentration being considered, the dose is lowered and the organism checked for survival.
Worms were picked onto fresh plates every alternate day to remove progeny, and were then checked for survival until all worms on the plates were dead.
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They were maintained for 4 to 5 days to allow them to fully reach the gravid stage and were checked daily for survival.
The animals were checked daily for survival.
The mice were checked daily for survival.
Flies were checked daily for survival and medium was replaced once every 2 weeks, as in previous experiments assessing longevity in this species [ 16, 36].
Worms were checked daily for survival and data were censored for nematodes that desiccated after crawling onto the side of a plate, which became more frequent in increasingly stressful conditions (Results).
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