Sentence examples for checked for successful from inspiring English sources
PCR reaction mixtures were initially checked for successful amplification on a 1.5 % agarose gel stained with ethidium bromide, and viewed under UV light.
The PCR products were cleaned with the Invisorb Spin PCRapid Kit (Invitek) or Quick-Clean (Bioline) and checked for successful amplification by running an aliquot on a 1.5% agarose gel, stained with 1.3 × 10-4 mg/ml ethidium bromide.
Membranes were checked for successful protein transfer and loading control by incubation of the membranes for 1 min in ponceau S. The MS-MLPA is a semi-quantitative method for methylation profiling using a methylation-sensitive restriction enzyme (Nygren et al, 2005).
After ESI-MS analysis to check for successful oxidation, the peptide was column purified and lyophilized.
A minus RT control with primers for GAPDH was performed to check for successful removal of all the contaminating DNA.
The PCR products were electrophoretically separated on 2% agarose gels and stained with ethidium bromide to check for successful amplification.
To check for successful deletion, we performed two different control PCRs, using primers csfDEL5F and csfDEL3R as well as ENVSP1F and ENVNEU2R (for primer sequences see Table 1).
The rats (n=5 in each group) were anesthetized by 3% isoflurane; loss of righting reflex, unresponsiveness and corneal reflexes were used to check for successful anesthesia.
Furthermore, we will use the Credibility/Expectancy Questionnaire (CEQ; [ 42]; Dutch translation: Godfrin et al.: CEQ-NL, unpublished document) to check for baseline group differences in treatment credibility/expectancy and to check for successful blinding of participants (post-training).
A PCR for the Equus caballus β-actin gene (Forward 5'-GAGCAAGAGGGGCATCCTGA-3', Reverse 5'-GGTCATCTTCTCGCGGTTGG-3', Gene ID: 100033878) was performed on each sample of cDNA to check for successful retro-transcription.
