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Campylobacter spp. and Yersinia ruckeri primers probes sets were designed and checked for specificity with diverse Campylobacter, related organisms, and other bacterial pathogens before being used in field samples.
The SEZ6L2-specific sequence (MHS1011-59266) was obtained from OpenBiosystem Huntsvillee, AL), sequenced for verification, and checked for specificity with BLAST against the human genome.
The probes were checked for specificity with NCBI Blast [ 36].
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For phospho-specific antibodies, each antibody was checked for specificity using cell extracts with and without appropriate ligand induction.
Primers were checked for specificity using NCBI BLAST searches.
All primer pairs (Table 1) were checked for specificity using BLAST analysis and were checked by both agarose gel electrophoresis and thermal dissociation curves to ensure amplification of a single product with the appropriate size and melting temperature.
All sequences used were checked for specificity by BLAST searches against genome databases.
All quantitative PCR results were checked for specificity by melting curve analysis.
PCR products were checked for specificity on a 2% agarose gel.
All samples were checked for specificity of amplification via Melting curve analysis.
The oligonucleotides were checked for specificity using the BLAST feature of SGD.
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