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For phospho-specific antibodies, each antibody was checked for specificity using cell extracts with and without appropriate ligand induction.
Gene-specific primers were designed and checked for specificity using Primer Premier 5.0 (Additional file 4: Table S4) and the NCBI primer Blast tool, respectively.
Schistosoma mansoni W specific primers pairs (SmWSPP, Additional file 2 table S1) were designed in the female-specific regions using Primer 3 http://frodo.wi.mit.edu/ and checked for specificity using Primer-Blast http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi.
Campylobacter spp. and Yersinia ruckeri primers probes sets were designed and checked for specificity with diverse Campylobacter, related organisms, and other bacterial pathogens before being used in field samples.
Primers were checked for specificity using NCBI BLAST searches.
All sequences used were checked for specificity by BLAST searches against genome databases.
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While checking for specificity of the PCR assay, the reactions with the templates form C. botulinum type C and C/D which were tested became positive, but the rest of the reactions turned negative.
Each primer was blasted against the whole Vitis genomic database to check for specificity.
A melt curve was also performed after the assay to check for specificity of the reaction.
Resulting amplicons were sequenced in order to check for specificity.
Resulting amplicons were sequenced to check for specificity.
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