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Proteins were checked for purity by Coomassie brilliant blue staining of 10 or 15% SDS-PAGE gels.
It was checked for purity by both 1H NMR and HPLC analysis, with material that was >98% pure taken onto animal studies.
The isolates were checked for purity and preserved at −70°C for further studies.
The obtained PCR products were checked for purity and size on 1.5% agarose gel.
Cultures were checked for purity by Gram staining and observed under a microscope at 1000 ×.
Isolates obtained were further streaked on Luria Bertani (LB) agar plates and checked for purity prior to subsequent molecular analysis.
The strains were checked for purity and bacterial counts showed that 24 h incubation at room temperature in UHT milk resulted in approximately 108 cfu mL−1.
RNA was quantified and checked for purity using a spectrophotometer (Nanodrop) at 260 nm.
Clostridia stock cultures were checked for purity on egg yolk agar (EYA) plates (Oxoid, Basingstoke, England).
Samples were quantified and checked for purity using an Ultrospec 2100 pro spectrophotometer (GE healthcare) and agarose gel electrophoresis.
After extraction, DNA was quantified by spectrophotometery, checked for purity and stored at −35°C until further analyses.
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