Sentence examples for checked for orientation from inspiring English sources

Each fragment was checked for orientation by using KS2808 and the fragment-specific reverse primer.

Samples were checked for orientation and inclusion of AF and NP, and scored for degree of degeneration using a published histological 12 point scale [ 24].

In the analysis of 16 S rDNA sequences, the bidirectional contigs were checked for orientation and sequence quality, and only the ones with correct primer sequences and one-way read length above 900 bp were accepted for further analyses.

The SPRY4 3′-UTR lucifereporterorter was constructed as follows: the full-length 3′-UTR of SPRY4 (947 nucleotides) was amplified using cDNA from RD cells (forward: 5′-AGAAGCCTGTTTCTCCGTACA-3′, reverse: 5′-TCAGAAAGGCTTGTCGGG-3′). The primers were digested and cloned into the Xba1 site of pGL3 (Promega, Madison, WI, USA), checked for orientation, sequenced, and named Wt.

The clone was checked for correct orientation of the insert by restriction enzyme digestion and confirmed by sequencing.

After being checked for proper orientation using PCR, the plasmid was digested with NotI, and linear insert DNA was prepared (Figure S2A) and microinjected into the pronuclei of BDF1 (C57BL/6 × DBA/2) mouse zygotes.

By manual inspection, the binding region was checked for the orientation of Ensembl known transcripts relative to the probe set.

These BACs were further screened for matches to the same chromosome in G. aculeatus and then checked for consistent orientation and distance.

Plasmids were checked for the presence and orientation of the insert by PCR, and those plasmids that contained an insert in the correct orientation were used to transform F62.

The positive clones were checked for the correct sequence and orientation by restriction enzyme digestion and by sequencing.

All constructs were checked for fidelity as well as number and orientation of integrated spacers by sequencing.