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Cells were regularly checked for mycoplasma contamination (MycoAlert Mycoplasma Detection kit; Lonza, Rockland, USA).
Cells were checked for mycoplasma contamination.
All cell lines were passaged for less than six months continuously and were routinely checked for mycoplasma contamination.
Cultures were regularly checked for Mycoplasma contamination.
Cell lines were routinely checked for mycoplasma.
Cells were routinely checked for mycoplasma contamination.
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Quality controls were made every 8 weeks to check for Mycoplasma contamination, ploidy, immunophenotype and cell morphology.
Blood and throat swabs of animals were checked for the genus mycoplasma by culture and PCR in order to determine that rabbits were mycoplasma free.
Cell lines were routinely checked for contamination by mycoplasma using Hoechst staining and consistently found to be negative.
In the present study we took great care to standardize the experimental procedures by using only freshly thawed B16-F10 cells (obtained from ATCC) of the same passage, which were regularly checked for viability and mycoplasma.
The Cells were grown as monolayers in RPMI medium supplemented with 10% foetal calf serum, 2 m M glutamine, 100 units per ml penicillin and 100 μg ml−1 streptomycin (Invitrogen, Cergy-Pontoise, France), at 37 °C in a humidified 5% CO2 atmosphere, and regularly checked for absence of mycoplasma.
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