Each node is tagged either as a source, internal or sink, and each edge is checked for preferential flow directions.
Perks and colleagues incubated Hs578T breast cancer cells with apoptotic inducers such as ceramide and RGD-containing peptide, and then checked for apoptosis using flow cytometry, the 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) assay, and morphological assessment [ 104].
Preventive strategies of DC dysfunction include ultrasound guidance for a higher chance of DC placement [5], checking for adequate flow immediately after placement, flushing each DC lumen with saline at the beginning and end of each RRT session, anticoagulant locking followed by careful clamp closing, and checking the correct position with x-rays.
Preventive strategies of DC dysfunction include ultrasound guidance for a higher chance of DC placement [ 5], checking for adequate flow immediately after placement, flushing each DC lumen with saline at the beginning and end of each RRT session, anticoagulant locking followed by careful clamp closing, and checking the correct position with x-rays.
Check for coherent flow and transitions.
Pull the radiator cap to check for adequate flow or leaks before it gets too hot.
Initially, the pump geometry was designed and grid-dependency test (Fig. 2), check for turbulence model and validity of the CFD simulations were checked for water at design flow condition.
To investigate the effect of PDT on cell viability, we checked for apoptosis or necrosis using flow cytometry analysis with Annexin V and PI.
To study whether the interaction of VDR with the HLA DRB1*0301 is through VDRE present in the promoter region of the HLA-DRB1*0301, we stimulated the International Histocomopatibility Workshop's DRB1*0301 homozygous B-lymphoblastoid cell line VAVY (B-LCL IHW09023) and DUCAF (B-LCL IHW09019) with 100 nM of calcitriol and checked for the expression of DR using flow cytometry and real time PCR.
The cultured cells were harvested and checked for c- myc expression by flow cytometry, RT PCR and Western blot.
Spirograms and flow-volume loops were checked for quality by an experienced technician from the University Hospital Leuven.
