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In addition, we found that after each passage, harvested bacteria must be cloned and checked for expression of the bioengineered gene to counter selection in favor of antigen loss mutants.
Transfected cells were checked for expression of GFP by fluorescence microscopy and harvested using a cell scraper.
Individual clones were checked for expression of the NhttEGFP transgene by microscopy and Western blot analysis following induction with CuSO4.
We also checked for expression of PSD-95 which is a post-synaptic density protein, important for formation of functional synapses.
After retroviral infection and selection with puromycin, the polyclonal populations of knockout cells reconstituted with MEKK1 were checked for expression levels in comparison to the DT40 cell line by western blot (Figure 5A, left panel).
Colonies were grown and checked for expression of the GTPases western blot analysis as explained above.
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To check for expression of APJ in human AVICs, western blotting and immunohistochemical method were performed.
RNA was extracted from a subset of transformed roots for genes listed in Additional file 2: Table S2 to check for expression of the Arabidopsis gene by PCR.
After reaction, remove 1 μl and add it to 3 μl 2X Laemmli buffer for analysis to check for expression of the protein.
Then, the RT Profiler PCR Array (SABioscience) was performed by real-time PCR using an ABI 7900HT Sequence Detection System to check for expression of inflammatory-related genes (Supplementary Tables 1 3).
These primers were also used to check for expression of genes involved in ethylene synthesis and signaling in dry wild-type (Col) and fus3 immature seeds.
More suggestions(16)
inspected for expression
evaluated for expression
characterised for expression
verified for expression
investigation for expression
monitored for expression
checked for colorectal
checked for radiation
checked for normality
checked for fate
checked for consistency
checked for homogeneity
checked for plagiarism
checked for influenza
checked for proportionality
checked for salt
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