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The techniques involve various parameters to be checked for effective output.
Before proceeding to microarray analysis each replicate set was checked for effective FGF inhibition.
Independent runs were checked for effective sample sizes (ESS) of more than 200 and convergence of posterior values in Tracer v1.5 (Rambaut and Drummond 2007), and convergence of tree topologies in FigTree v1.3.1 (Rambaut 2009) before combining.
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After initial acquisition Triton X-100 (0.2 %) was added to each well and 10 min later cells were visualised to check for effective PI staining and removal of Calcein-AM staining.
Single runs were combined with Log-Combiner 1.5.3 and checked for sufficient effective sampling size (ESS) in Tracer 1.5.3.
We checked for the effective sample size (ESS) convergence and the stationary of the different analyses in Tracer 1.4 [ 92] and combined the results in the BEAST module LogCombiner 1.4.4.
Independence among samplings was confirmed for each run by checking for high effective sample sizes (>200).
We checked for stationary posterior distributions, effective sample sizes (ESS) above 200, and convergence between runs by examining parameter traces with the program Tracer v1.6 [ 49].
The runs were checked for quality and sufficient ESS (effective sample size) in Tracer 1.5.3.
The influence of the charge and molecular size of the peptide on its effective mobility was checked for different semiempirical models.
and checked for errors.
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