Outliers will be checked for errors by comparison with original data collection forms, whenever possible.
Each published mutation was checked for accuracy by comparison to the GNAS wild-type sequence.
The products were sequenced and checked for mutations by comparison with reference databases (NM_000222 for c-kit, NM_006206 for PDGF-R α, NM_002609 for PDGF-R β).
The RNA was quantified and checked for purity by comparison of absorbance at 260 and 280 nm in the NanoDrop Spectrophotometer (Thermo Scientific, Wilmington, DE, USA).
Clinical and laboratory data were recorded on paper-based case record forms and subsequently entered into an Access 2003 database (Microsoft, Redmond, WA, USA) and systematically checked for errors by comparison with the original case record forms.
After retroviral infection and selection with puromycin, the polyclonal populations of knockout cells reconstituted with MEKK1 were checked for expression levels in comparison to the DT40 cell line by western blot (Figure 5A, left panel).
Check for other comparisons that don't use "like" or "as," and underline them also.
For 12 such loci (picked at random, except for avoiding short loci, <300 bp), the sequences were aligned using Clustal Omega (Sievers and Higgins, 2014) and checked for presences of introns by comparison to the bean genome (Schmutz et al., 2014; http://www.phytozome.net/commonbean.php); degenerate primers were then designed to flank the introns using Primaclade (Gadberry et al., 2005; Table 2).
All variables were checked for normal distribution and multiple comparisons were performed by paired t-test or one-way analysis of variance (ANOVA).
In order to ensure the validity of results obtained, the pairwise comparison matrices were checked for consistency.
